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Recombinant Human Ephrin type-A receptor 2 (EPHA2), partial (Active)
SPP-01649
| Size | Price |
| 1 mg | Online Inquiry |
| 500 μg | Online Inquiry |
| 50 μg | Online Inquiry |
| 10 μg | Online Inquiry |
| Target Information | |
|---|---|
| Species | Homo sapiens |
| Target Name | EphA2 |
| UniProt No. | P29317 |
| Subcellular Location | Cell membrane, Single-pass type I membrane protein, Cell projection, ruffle membrane, Single-pass type I membrane protein, Cell projection, lamellipodium membrane, Single-pass type I membrane protein, Cell junction, focal adhesion |
| Tissue Specificity | Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary. |
| Gene Abbr. | EPHA2 |
| Full Name | EPH receptor A2 |
| Alias | ARCC2, CTPA, CTPP1, CTRCT6, ECK |
| Introduction | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer. Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered. The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells.The EphA3 receptor preferentially binds ephrin-A5. This ligand-receptor interaction stimulates EphA3 signaling, regulates cell adhesion and migration, and induces cellular morphologic responses. EphA3 plays a critical role in callosal axon guidance retinotectal mapping of neurons as well as cardiac cell migration and differentiation. Investigators have shown that somatic mutations in functional domains of EphA3 are linked to lung cancer progression. In addition, EphA3 expression levels have been correlated with tumor angiogenesis and progression in gastric and colorectal carcinoma.Both Tyr602 and Tyr779 phosphorylation are involved in ephrin-A5 induced EphA3 receptor activation. Phosphorylated Tyr779 of the EphA3 receptor is the binding site for the SH2-domain-containing Crk adaptor, which in turn activates the small GTPase RhoA. |
| Product Details | |
|---|---|
| Product Type | Recombinant Protein |
| Product Form | Lyophilized powder |
| Buffer before Lyophilization | Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4. |
| Storage & Handling | |
|---|---|
| Storage Temp. | Store at -20 °C upon receipt unless otherwise instructed. |
| Handling | Aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.