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Recombinant Human Death-associated protein kinase 1 (DAPK1), partial
SPP-01459
| Size | Price |
| 20 μg | Online Inquiry |
| 100 μg | Online Inquiry |
| 500 μg | Online Inquiry |
| Target Information | |
|---|---|
| Species | Homo sapiens |
| Target Name | DAPK1 |
| UniProt No. | P53355 |
| Subcellular Location | Isoform 1: Cytoplasm, Cytoplasm, cytoskeleton |
| Tissue Specificity | Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. |
| Gene Abbr. | DAPK1 |
| Full Name | death associated protein kinase 1 |
| Alias | DAPK, ROCO3 |
| Introduction | Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain. Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death. The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1.Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia. |
| Product Details | |
|---|---|
| Product Type | Recombinant Protein |
| Storage & Handling | |
|---|---|
| Storage Temp. | Store at -20 °C upon receipt unless otherwise instructed. |
| Handling | Aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.