RBPJ cDNA ORF Clone, Human, untagged - CD BioSciences

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RBPJ cDNA ORF Clone, Human, untagged

RBPJ cDNA ORF Clone, Human, untagged

SPD-13150

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Description
Full length Clone DNA of Human recombination signal binding protein for immunoglobulin kappa J region.
Target Information
Species Human
Target Name RBPSUH
Gene Abbr. RBPJ
Gene ID 3516
Full Name recombination signal binding protein for immunoglobulin kappa J region
Alias AOS3, CBF-1, CBF1, IGKJRB, IGKJRB1
Introduction RBPSUH (Recombining Binding Protein, SUppressor of Hairless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes. RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells.
Product Details
Description Full length Clone DNA of Human recombination signal binding protein for immunoglobulin kappa J region.
NCBI Ref Seq NM_005349.2
RefSeq ORF Size 1503 bp
Vector pCMV3-untagged
Promoter Enhanced CMV promoter
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Ampicillin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.