Ras Antibody - CD BioSciences

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Ras Antibody

Ras Antibody

SPA-09507

Size Price
100 µL Online Inquiry
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Target Information
Target Name Ras
Gene Abbr. KRAS
Gene ID 3845
Full Name KRAS proto-oncogene, GTPase
Alias 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B
Introduction The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
Product Details
Host Rat
Clonality Monoclonal
Clone No. 342404
Isotype IgG2B
Immunogen E. coli-derived recombinant human K-Ras2B. Met1-Met188 (Gln61His). P01116-2.
Usage
Application WB
Dilutions Western Blot (5 µg/mL)
MW(KDa) 21
Reactivity Human
Specificity Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western Blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.