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Ras Antibody
SPA-09507
Size | Price |
100 µL | Online Inquiry |
More Options | Online Inquiry |
Target Information | |
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Target Name | Ras |
Gene Abbr. | KRAS |
Gene ID | 3845 |
Full Name | KRAS proto-oncogene, GTPase |
Alias | 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B |
Introduction | The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. |
Product Details | |
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Host | Rat |
Clonality | Monoclonal |
Clone No. | 342404 |
Isotype | IgG2B |
Immunogen | E. coli-derived recombinant human K-Ras2B. Met1-Met188 (Gln61His). P01116-2. |
Usage | |
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Application | WB |
Dilutions | Western Blot (5 µg/mL) |
MW(KDa) | 21 |
Reactivity | Human |
Specificity | Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western Blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb. |
Storage & Handling | |
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Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | No Preservative |
Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.