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Ras Antibody
SPA-09505
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Ras |
| Gene Abbr. | KRAS |
| Gene ID | 3845 |
| Full Name | KRAS proto-oncogene, GTPase |
| Alias | 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B |
| Introduction | The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.Additional oncogenic mutations of Ras have been observed at varying frequencies at codons 12, 13, and 61. The Gly12 to Val12 (G12V) mutation has been detected in a number of different cancers, including colorectal and thyroid cancer. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (1:500-1:10000); Immunohistochemistry (1:100-1:1000) |
| MW(KDa) | 21 |
| Reactivity | Human, Rat, Zebrafish |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (pH 7), 20% Glycerol. |
| Preservative | 0.025% Proclin 300 |
| Storage Temp. | Aliquot and store at -20 °C or -80 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.