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Rad51 Antibody
SPA-09450
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Rad51 |
| Gene Abbr. | RAD51 |
| Gene ID | 5888 |
| Full Name | RAD51 recombinase |
| Alias | BRCC5, FANCR, HRAD51, HsRad51, HsT16930 |
| Introduction | DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination. Research studies have shown that defects in both pathways are associated with human disease, including cancer.DSBs that arise during S or G2 phase are repaired via homologous recombination using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2. BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR.Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk. Some studies have implicated Rad51 as a potential marker for pancreatic cancer. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D4B10 |
| Immunogen | Recombinant protein specific to full-length human Rad51 protein. This antibody recognizes residues surrounding Leu140 of human Rad51. |
| Source/Purification | Antibody is produced by immunizing animals with recombinant protein specific to full-length human Rad51 protein. This antibody recognizes residues surrounding Leu140 of human Rad51. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 37 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total Rad51 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.