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RACK1 Antibody
SPA-09447
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | RACK1 |
| Gene Abbr. | RACK1 |
| Gene ID | 10399 |
| Full Name | receptor for activated C kinase 1 |
| Alias | GNB2L1, Gnb2-rs1, H12.3, HLC-7, PIG21 |
| Introduction | The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms. RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways. One example of this is its enhancement of PKC-dependent JNK activation. RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well. RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D59D5 |
| Immunogen | Synthetic peptide corresponding to central residues of human RACK1 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 32 |
| Reactivity | Human, Mouse, Rat, Monkey, D. melanogaster |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total RACK1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.