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Rac1 Antibody
SPA-09438
| Size | Price |
| 0.1 mL | Online Inquiry |
| 0.025 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Rac1/cdc42 |
| Gene Abbr. | RAC1 |
| Gene ID | 5879 |
| Full Name | Rac family small GTPase 1 |
| Alias | MIG5, MRD48, Rac-1, TC-25, p21-Rac1 |
| Introduction | Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state.A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay. Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthesized non-phosphopeptide derived from human Rac1 around aa. 71 (P-L-S-Y-P). |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1:500-1:1000); Immunofluorescence (1:100-1:200) |
| MW(KDa) | 21 |
| Reactivity | Human, Mouse, Rat |
| Specificity | Rac1 (specific to a.a. 71) detects endogenous levels of total Rac1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.