POLE3 Knockout Cell Line - CD BioSciences

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POLE3 Knockout Cell Line

POLE3 Knockout Cell Line

SPL-02670

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1 Unit Online Inquiry
Description
Frameshift mutation
Target Information
Target Name POLE3
Gene Abbr. POLE3
Gene ID 54107
Full Name DNA polymerase epsilon 3, accessory subunit
Alias CHARAC17, CHRAC17, CHRAC2, YBL1, p17
Species Human
WT Expression Level 107.52 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a frameshift mutation in a coding exon of POLE3.
Description Frameshift mutation
Parental Cell Line C631
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.