POLE Knockout Cell Line - CD BioSciences

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POLE Knockout Cell Line

POLE Knockout Cell Line

SPL-02669

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1 Unit Online Inquiry
Description
8bp deletion
Target Information
Target Name POLE
Gene Abbr. POLE
Gene ID 5426
Full Name DNA polymerase epsilon, catalytic subunit
Alias CRCS12, FILS, IMAGEI, POLE1
Species Human
Genomic Locus chr12:132681239
Transcript NM_006231
WT Expression Level 46.82 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 8bp deletion in a coding exon of POLE.
Description 8bp deletion
Parental Cell Line C631
Guide RNA Sequence AGTTTCGGCACTCAAGCGCC
PCR Primer Forward: CACTCTTTAGATAAGGACCACGCTA
Reverse: AGAGCAAGACTCCGTCTCAAAAA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.