Xylose

Inquiry

Cat: PA00589

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Higher plants and algae

Datasheet:

Product Info

Immunogen

xylose residues bound to the N-glycan in beta 1,2

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 µg/ml (ELISA), 2 µg/10 ml incubation buffer (WB)

Expected | apparent MW

10-100 for various glycoproteins

Reactivity

Confirmed reactivity

Higher plants and algae

Predicted reactivity

Higher plants

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.,Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385. Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Description

The antibody specifically cross-reacts with xylose residues in β1,2 that bind to the protein N-glycan. This residue is characteristic of plant protein N-glycans and is not present in animal protein N-glycans. This residue is added to the Golgi apparatus.

For research use only, not for clinical use.