WHIRLY2

Inquiry

Cat: PA01365

Size: 50 µg

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Hordeum vulgare

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI

Host

Chicken

Clonality

Polyclonal

Purity

Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.

Format

Liquid

Storage

Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunogold (IG), Western blot (WB)

Recommended dilution

1: 100 (IG), 1: 1500 (WB)

Expected | apparent MW

25 kDa

Reactivity

Confirmed reactivity

Hordeum vulgare

Predicted reactivity

Brachypodium distachyon, Triticum urartu

Not reactive in

Glycine max

Description

The WHIRLY protein is a multifunctional DNA/ rna binding protein (Prikryl et al. 2008, NAR 36:5152-5165). Studies have shown that WHIRLY1 is present in both chloroplasts and nuclei (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts, it is part of the nucleoid (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, plant 232:471-481). In the nucleus, WHIRLY1 is an activator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein that affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).

For research use only, not for clinical use.