VRN2 | Vernalization protein 2

Inquiry

Cat: PA00955

Size: 50 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Triticum aestivum

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Host

Mouse

Clonality

Monoclonal

Purity

Total IgG. Protein G purified purified from Cell culture supernatant.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB)

Recommended dilution

1 : 10 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

23,7 kDa

Reactivity

Confirmed reactivity

Triticum aestivum

Predicted reactivity

Triticum monococcum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

VRN-2 and ZCCT2 full-length proteins are expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins are used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody is able to differentiate VRN-2 from the ZCCT2 protein.,There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum

Description

Plants with winter growth habits will bloom prematurely after being exposed to low temperatures for several weeks, a process known as vernalization. Wheat vernalization gene VRN-2 is a dominant flowering suppressor and is down-regulated during vernalization. Loss of function of VRN-2, whether naturally mutated or absent, results in vernal lines that do not require vernalization to flower.

For research use only, not for clinical use.