VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)
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VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)

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Cat: PA01132
Size: 200 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
Recombinant, His6 tagged VPS35b of Arabidopsis thaliana UniProt: F4I0P8, TAIR: At1g75850
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format
Liquid at 2 mg/ml.
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
assay dependent (ELISA), 1: 400 (IF), 1: 100 (IP), 1: 1000 (WB)
Expected | apparent MW
89 | 98 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Description

VPS35(vacuolar protein Sorting Related Protein 35) plays a role in vesicular protein sorting. A component of membrane-associated reverse transcription complexes essential in retrograde kernal to Golgi transport. It also participated in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in the recycling of specific cargo from the endosome to the plasma membrane. Arabidopsis has three VPS35 homologues, VPS35a, VPS35b, and VPS35c, of which VPS35b plays the most important role in these processes. Positioned in front cavitation chamber (PVC).

For research use only, not for clinical use.