Vicia Villosa Lectin (VVL, VVA), Biotinylated

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Cat: PL00747

Size: 2 mg

Sugar Specificity: N-Acetylgalactosamine

Datasheet:

Description

The binding specificity of Wisteria floribunda lectin (WFL) is not fully understood, but this lectin appears to preferentially bind to carbohydrate structures that terminate when n-acetylgalactosamine connects alpha or beta to position 3 or 6 of galactose. This lectin has been used to separate lymphocyte populations and, while not mitotic, can induce lymphokine production in mouse spleen cells.

Product Info

Conjugate

Biotinylated

Concentration

2 mg active conjugate/ml
5 mg active conjugate/ml

Sugar Specificity

N-Acetylgalactosamine

Storage

2-8℃; Store frozen for long term storage

Application

Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology

Recommended dilution

For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.

For research use only, not for clinical use.