Vicia Villosa Lectin (VVL, VVA), Agarose bound

Inquiry

Cat: PL00750

Size: 2 ml

Sugar Specificity: N-Acetylgalactosamine

Datasheet:

Description

Agarose bound villus lectins are prepared using our affinity purified lectins. This lectin is a family of tetramer glycoproteins consisting of a combination of A and B subunits with a structure similar to that of PHA and GSLi. In our preparation, the main isolates appear to be rich in B subunits. VVL prioritises the recognition of α- or β-linked terminal N-acetylgalactosamine, particularly individual α-n-acetylgalactosamine residues linked to serine or threonine in the polypeptide (Tn antigen).

Product Info

Conjugate

Agarose

Sugar Specificity

N-Acetylgalactosamine

Storage

2-8℃; Do not freeze

Application

Glycobiology, Affinity Chromatography

Recommended dilution

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide

For research use only, not for clinical use.