VDE | Violaxanthin de-epoxidase
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VDE | Violaxanthin de-epoxidase

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Cat: PA00390
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Brachypodium distachyon, Deschampsia antarctica, Oryza sativa,
Datasheet:

Product Info

Immunogen
His-tagged, recombinant VDE from Arabidopsis thaliana, overexpressed in E.coli, Uniprot:Q39249 , TAIR: AT1G08550
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
Propeptide 52 kDa, mature protein: 39.7 kDa, migrates at 40 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Brachypodium distachyon, Deschampsia antarctica, Oryza sativa,
Predicted reactivity
Brassica napus, Camellia sinensis, Citrus limon, Cucumis sativus, Chrysanthemum morifolium, Citrus sinensis, Coffea arabica, Fragaria ananassa, Glycine soja, Gossypium arboreum, Lactuca sativa, Lycium barbarum, Morus notabilis, Nicotiana tabacum, Phyllostachys edulis, Picea abies, Prunus humilis, Spinacia oleracea, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Zingiber officinale, Vitis vinifera
Not reactive in
diatoms

Additional information

Extraction buffer must contain 7 M urea for Brachypodium distachyon and Oryza sativa samples.This product can be sold containing ProClin if requested.

Description

Violaxanthin deepoxidase (VDE) is part of the lutein (or violaxanthin) cycle and is used to control zeaxanthin concentrations in chloroplasts. This enzyme catalyzes a two-step single deepoxidation reaction. Zeaxanthin induces excitation energy dissipation in chlorophyll of the photoharvesting protein complex of photosystem II.

For research use only, not for clinical use.