V-ATPase holoenzyme

Inquiry

Cat: PA01672

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Avena sativa

Datasheet:

Product Info

Immunogen

V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 400 - 1: 1000 (WB)

Expected | apparent MW

100, 44 , 42, 36 , 16, 13 kDa

Reactivity

Confirmed reactivity

Avena sativa

Predicted reactivity

higher plants

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin

Description

Plant vacuole V-ATPase is responsible for energy transport of ions and metabolites and acts as a "steward" and stress response enzyme. V-atpase is a multisubunit enzyme composed of membrane region and cytoplasmic catalytic region. It is related to FoF1 ATP synthase. Name of replacement protein: vacuolar proton pump subunit E, protein embryo defect 2448er type CA2+ -ATPase 1,ATECA1, ECA1, er type CA2+ -ATPase 1.

For research use only, not for clinical use.