V-ATPase | Epsilon subunit of tonoplast H+ATPase

Cat: PA01563

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1, TAIR: At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution

1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Expected | apparent MW

26 | 31 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera

Predicted reactivity

Brachypodium dystachyon, Capsella rubella, Chenopodium quinoa, Citrus clementina, Citrus unshiu, Citrus limon, Eucalypsus grandis, Glyxine max, Glycine soja, Lotus japonicus, Phaseolus sp. , Physcomitrium patens, Populus trichocarpa, Prunus persica, Ricinus communis, Riticum aestivum, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor Theobroma cacao, Vitis vinifera, Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat

Not reactive in

Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Additional information

Cellular [compartment marker] of tonoplast membrane.This product can be sold containing ProClin if required.,V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample. Immunostaining protocol using V-ATPase antibodies can be found here.

Description

Plant vacuole V-ATPase is responsible for energy transport of ions and metabolites and acts as a "steward" and stress response enzyme. V-atpase is a multisubunit enzyme composed of membrane region and cytoplasmic catalytic region. It is related to FoF1 ATP synthase. Replacement protein name: vacuolar proton pump subunit E, protein Embryo defect 2448

For research use only, not for clinical use.