V-ATPase, c | Vacuolar H+-ATPase, subunit c (16 kDa)
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V-ATPase, c | Vacuolar H+-ATPase, subunit c (16 kDa)

Cat: PA00586
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c UniProt: Q6IDA4, TAIR: AT2G16510
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
16 | 16 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum
Predicted reactivity
dictos including: Cucumis sativus, Gossypium mexicanum, Manihot esculenta, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
Not reactive in
Algae

Additional information

Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Description

The V-ATPase c subunit is located in the vacuole and is involved in ATP synthesis and proton transport. The protein is encoded by the ATVHA-C3 gene. Alternative name :AT4g34720/T4L20_300

For research use only, not for clinical use.