Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel
The V-ATPase c subunit is located in the vacuole and is involved in ATP synthesis and proton transport. The protein is encoded by the ATVHA-C3 gene. Alternative name :AT4g34720/T4L20_300
For research use only, not for clinical use.