V-ATPase, B | vacuolar H+-ATPase subunit B
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V-ATPase, B | vacuolar H+-ATPase subunit B

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Cat: PA00585
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis halleri, Nicotiana tabaccum, Thellungiella salsuginea, Vigna radiata
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
53 | 57 kDa (Vigna radiata)

Reactivity

Confirmed reactivity
Arabidopsis halleri, Nicotiana tabaccum, Thellungiella salsuginea, Vigna radiata
Predicted reactivity
Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Cucumis sativus, Glycine soja, Gossypium mexicanum, Halostachys caspica,  Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrium patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
Not reactive in
Thermatoga neapolitana

Additional information

Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Description

V-atpase, subunit B is a non-catalytic subunit of the vacuolar ATpase peripheral V1 complex. Nicknames: vacuole proton pump B1 subunit, vacuole H+ -ATPase B subunit, V-ATPase 57kda subunit.

For research use only, not for clinical use.