Urease, Biotin conjugated

Inquiry

Cat: PA00693

Size: 10 mg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Canavalia ensiformis

Datasheet:

Product Info

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host

Rabbit

Clonality

Polyclonal

Purity

Purified IgG in PBS.

Format

Lyophilized

Reconstitution

For reconstitution add 1 ml of sterile destilled water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Recommended dilution

1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW

91 kDa

Reactivity

Confirmed reactivity

Canavalia ensiformis

Predicted reactivity

Arabidopsis thaliana, Glycine max, Ricinus communis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition,Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Description

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

For research use only, not for clinical use.