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Ulex Europaeus Agglutinin I (UEA I), Agarose bound
Cat: PL00652
Size: 2 ml
Sugar Specificity: Fucose
Datasheet:
Description
Agarose binding iris lectin is prepared from affinity purified lectin. Macroalgin I binds to many glycoproteins and glycolipids containing α-linked focal residues, such as ABO blood group glycoconjugates. This lectin binds preferentially to blood type O cells and has been used to determine secretion status. It has been identified as an excellent marker for human endothelial cells.
Product Info
Conjugate
Agarose
Sugar Specificity
Fucose
Storage
2-8℃; Do not freeze
Application
Glycobiology, Affinity Chromatography
Recommended dilution
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide
For research use only, not for clinical use.