UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)

Cat: PA01847

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Arabidopsis halleri, Brassica rapa, Capsicum annuum, Fortunella margarita Swingle, Citrus sinensis, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, Medicago truncatula, Mesemryanthenum crystallinum, M.vaginalis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivu, Zea mays

Datasheet:

Product Info

Immunogen

Recombinant UGPase Q43772 overexpressed and purified from E.coli

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunolocalization (IL), Western blot (WB)

Recommended dilution

1 : 1500 (IL), 1 : 1000-1 : 5000 (WB)

Expected | apparent MW

51,6 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Arabidopsis halleri, Brassica rapa, Capsicum annuum, Fortunella margarita Swingle, Citrus sinensis, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, Medicago truncatula, Mesemryanthenum crystallinum, M.vaginalis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivu, Zea mays

Predicted reactivity

Amorpha fruticosa, Bambusa oldhamii, Brachypodium distachyon, Brassica pekinensis, Capsella rubella, Cucumis melo, Eucalyptus grandis, Glycine max, Glycine soja, Gossipium hirsutum, Jatropha curcas, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Sorghum bicolor, Theobroma cacao, Zea mays, Vitis vinifera

Not reactive in

C. merolae, diatoms

Additional information

Cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)This product can be sold containing ProClin if requested.,This antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana.

Description

Glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. It is a key enzyme in the synthesis of sucrose, cellulose and other sugars. In Arabidopsis, UGPase-A has two cytoplasmic isomers (94% identity at the amino acid level) and UGPase-B has one chloroplast isomer with an identity of about 10-11% (Kleczkowski et al. 2011).

For research use only, not for clinical use.