UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker) (Hordeum vulgare)

Inquiry

Cat: PA00484

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays

Datasheet:

Product Info

Immunogen

His-tagged, full length Hordeum vulgare UGPase, overexpressed and purified from E.coli, UniProt: Q43772.1

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 10 000 (WB)

Expected | apparent MW

52 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Zea mays

Predicted reactivity

Bambusa oldhamii, Brassica pekinensis, Brassica rapa, Capsicum annuum, Cucumis sativus, Dendrobium catenatum, Dendrocalamus sinicus, Glycine max, Gossipium hirsutum, Lycopersicum esculentum, Lycopersicum chilense, Marchantia polymorpha, Oryza sativa, Picea glauca, Populus sp., Solanum tuberosum, Populus tremula, Ricinus communis, Saccharum officinarum, Vitis vinifera, for more species, please Species of your interest not listed inquire

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al, (2002),This antibody is also recognizing recombinant UGPase, below 0,5 pmol

Description

Glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. It is a key enzyme in the synthesis of sucrose, cellulose and other sugars. In Arabidopsis, UGPase-A has two cytoplasmic isomers (94% identity at the amino acid level) and UGPase-B has one chloroplast isomer with an identity of about 10-11% (Kleczkowski et al. 2011). Alternative name :UTP - glucose-1-phosphate uridine transferase.

For research use only, not for clinical use.