Tubulin gamma chain (monoclonal antibodies)
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Tubulin gamma chain (monoclonal antibodies)

Cat: PA00764
Size: 100 µg
Host: Mouse
Clonality: Monoclonal
Confirmed reactivity: Chlorella vulgaris, Nicotiana tabacum, human, protozoa
Datasheet:

Product Info

Immunogen
KLH-conjugated gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449. UniProt: P23258The epitope is located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Host
Mouse
Clonality
Monoclonal
Purity
Immunoglobulin Protein A purified in PBS. Contains 15 mM sodium azide.
Format
Liquid
Storage
Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
2-8 µg/ml (ICC) methanol/acetone fixation required, 1-2 µg/ml (WB)
Expected | apparent MW
51 kDa

Reactivity

Confirmed reactivity
Chlorella vulgaris, Nicotiana tabacum, human, protozoa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope which this antibody is recognizing is conserved in Arabidopsis thaliana Tubulin gamma-1 chain, UniProt: P38557, Gene ID: At3g61650 and Tubulin gamma-2 chain, UniProt: P38558, Gene ID:  At5g05620Recommended secondary antibody: goat anti-mouse IgG1 AS16 3715

Description

Tubulin gamma chains are essential for the control of microtubule networks. It is found in microtubule tissue centers (MTOC), such as spindle rods

For research use only, not for clinical use.