Tubulin beta chain
  1. Home
  2. Products
  3. Plant Antibodies
  4. Plant General Antibodies
  5. Loading Controls
Products
Online Inquiry

Tubulin beta chain

Inquiry
Cat: PA01012
Size: 100 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890).
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 1000 (IF), 1 : 500 (WB)
Expected | apparent MW
49 | 49 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Predicted reactivity
Brassica napus, Glycine max, Hordeum vulgare, Micromonoas pusilla, Nicotiana attenuata, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrium patens, Populus trichocarpa, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vigna radiata, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.,Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.

Description

Tubulin and tubulin together form microtubules.

For research use only, not for clinical use.