Tubulin alpha chain (monoclonal antibody)
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Tubulin alpha chain (monoclonal antibody)

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Cat: PA00763
Size: 100 µg
Host: Mouse
Clonality: Monoclonal
Confirmed reactivity: Arabidopsis thaliana, Chlorella vulgaris, Eisenia, human, mouse, Nicotiana tabacum, Paramecium sp. , pig, turkey, yeast
Datasheet:

Product Info

Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization, UniProt: Q71U36
Host
Mouse
Clonality
Monoclonal
Purity
Immunoglobulin Protein A purified in PBS. Contain 15 mM sodium azide.
Format
Liquid
Storage
Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Flow cytometry (FlowCyt), Immunocytochemistry (ICC), Immunohistochemistry on paraffin sections (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 500 (ICC), 1-4 µg/ml /FlowCyt), 1-4 µg/ml (WB)
Expected | apparent MW
51 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Chlorella vulgaris, Eisenia, human, mouse, Nicotiana tabacum, Paramecium sp. , pig, turkey, yeast
Predicted reactivity
Plants
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions, data in application example,,This antibody is recognizing defined epitope (amino acid 65-97) on N-terminal structural domain of alpha tubulin.Recommended secondary antibody, goat anti-mouse IgG1, HRP conjugated AS16 3715

Description

Microtubulin (TUA) and tubulin together form microtubules.

For research use only, not for clinical use.