TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)

Inquiry

Cat: PA00447

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana TrxM1 UniProt: O48737, TAIR: AT1G03680 and TrxM2 UniProt:Q9SEU8, TAIR: AT4G03520

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

20 | 14 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica napus, Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Populus balsamifera, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Theobroma cacao, Zea mays, Viola biflora

Not reactive in

Marchantia polymorpha, Physcomitrella patens

Additional information

5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.To work with this antibody chloroplast fraction has to be used.

Description

TrxM1/M2 (Thioredoxin M1/M2, chloroplasticT) is a thiol disulfide oxidoreductase involved in the REDOX regulation of both the reducing pentose phosphate pathway (Calvin-Benson cycle) and the oxidizing pentose phosphate pathway enzymes. Under reducing conditions, glyceraldehyde-3-phosphate dehydrogenase and urea phosphate kinase are activated and inhibited. Glucose-6-phosphate dehydrogenase. Activate NADP-malate dehydrogenase

For research use only, not for clinical use.