TROL | Thylakoid rhodanese-like protein

TROL protein can be used as a chloroplast dual-localization marker: for the thylakoids (the major portion of the protein) and the chloroplastic inner envelope. Since the envelopes make a very small portion of the total membranes, the envelope form of TROL is detectable only when isolated envelopes are applied. For AgriseraECLBright and Agrisera matching secondary antibodies goat anti-rabbit HRP conjugated, dilution 1: 25 000 1h/RT incubation, anti-TROL antibodies can be used in dilution of  1:3000.Recommendation: 2 µg chlorophyll/well. With increased protein or chlorophyll load/well background bands may be detected. Check here.

TROL(thylakoid Rodanase-like protein) is an indispensable membrane component in the photosynthetic organs of higher plants. TROL participates in the final step of photosynthetic electron transport by binding to the key energy converting enzyme ferridodoxin NADP+ REDOX reductase (FNR). This interaction causes the photosynthetic electrons to be transferred directly from the iron-thionein ferridoxin at the photosystem I matrix location to the FNR, where they are then handed over to NADP+. TROL is located in two different chloroplast chambers - in the inner envelope of the chloroplast, in its precursor form; In the thylakoid membrane, it is completely processed. Alternative names: thylakoid Rhodan-like 1, thiotransferase 4,AtStr4