tRNA-splicing endonuclease subunit Sen2-1 (SEN1), Recombinant Protein
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tRNA-splicing endonuclease subunit Sen2-1 (SEN1), Recombinant Protein

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Cat: RP01749
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana tRNA-splicing endonuclease subunit Sen2-1 (SEN1)
Product Gene Name
SEN1 recombinant protein
Product Synonym Gene Name
SEN1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES EEAELLDRCC FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS LENEVDLWRS MSSFKQDFAI LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH HPSLVHSEYA VLVQSIGGND RLKVWSDIHC SVRLTGSVAK SLLVLYVNRK VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT
Sequence Positions
1-237, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
27,359 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
tRNA-splicing endonuclease

NCBI/Uniprot Data

NCBI Accession #
NP_001078245.1
NCBI GI #
145332759
NCBI GenBank Nucleotide #
NM_001084776.2
NCBI GeneID
823701
NCBI Official Full Name
splicing endonuclease 1
NCBI Official Symbol
SEN1
NCBI Official Synonym Symbols
ATSEN1; splicing endonuclease 1
NCBI Protein Information
splicing endonuclease 1
NCBI Summary
Encodes a catalytic subunit of tRNA splicing endonuclease.
UniProt Gene Name
SEN1
UniProt Synonym Gene Names
AtSen1
UniProt Protein Name
tRNA-splicing endonuclease subunit Sen2-1
UniProt Synonym Protein Names
tRNA-intron endonuclease Sen2-1; AtSen1
UniProt Primary Accession #
Q9M1E8
UniProt Secondary Accession #
Q9S7L3
UniProt Related Accession #
Q9M1E8
UniProt Comments
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage .

For research use only, not for clinical use.