tRNA (guanine (37)-N1)-methyltransferase (Sb04g025390), Recombinant Protein
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tRNA (guanine (37)-N1)-methyltransferase (Sb04g025390), Recombinant Protein

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Cat: RP20154
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Sorghum bicolor (Sorghum) (Sorghum vulgare)
Datasheet:

Product Info

Full Product Name
Recombinant Sorghum bicolor tRNA (guanine (37)-N1)-methyltransferase
Product Gene Name
Sb04g025390 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEKLDESKFE QRLQLWALRI PRELSSAVTR LLRSGYLLDK PRVKTVVEDP EGDKNRLVIL SEKIQKPDLS DMPQQELDSL KQLCNVDVVP YTLTLGYSYW SAGHVAHLNI SDDLLPYKNV IAKVIYDKNY PRIQTVANKV GTITNEFRVP KFEILAGKND MVTEVKQYGA TFKLDYGLVY WNSRLDHEHI RLVSLFKKGD VICDMFAGIG PFAIPAAQKG CVVYANDLNP DSVHYLRTNA KINKVDDYIF AYNMDARVFM QNLMTVPGLE TGSDCQVAAD ESYPKEGVPA NENSSSNGNH NDVREGSQNG ANESSVASTT AKKRQQTSEE CESDCQDGDA SQTKRRNNKR VRGPGPPPSK PWEHFDHVLM NLPASALQFL DCFDGLVQKK YWTGSLPWIH CYCFIRSSES EESILSNKLN AKIAEPIFHR VRDVAPNKAM FCLSFKLPME CLREDDSENH IESVA
Sequence Positions
1-465, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
52,540 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
XP_002452416.1
NCBI GI #
242062254
NCBI GenBank Nucleotide #
XM_002452371.1
NCBI GeneID
8056501
NCBI Official Symbol
LOC8056501  
NCBI Protein Information
tRNA (guanine(37)-N1)-methyltransferase
UniProt Gene Name
Sb04g025390  
UniProt Protein Name
tRNA (guanine(37)-N1)-methyltransferase
UniProt Synonym Protein Names
M1G-methyltransferase
UniProt Primary Accession #
C5XX79
UniProt Comments
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

For research use only, not for clinical use.