Traditional quantitative methods are used to analyze samples of tissue or a large number of cells and obtain homogenized results, but they are not suitable for trace samples. Trace plant samples (<5000 cells) include cells with low protein content in the fields of early plant development and immunology research. Studying the intracellular protein abundance of trace plant samples is of great significance for plant growth, metabolism, and resistance processes.

CD BioSciences has advanced instruments, offering SMID and CBA technologies for quantitative protein analysis of trace plant samples. We also help our clients gain a deeper understanding of rare sample proteins.

Service Content

Quantification of trace plant samples protein based on SMID

The SMID is the most commonly used method for protein quantification in trace plant samples. This method can extract protein peptides in one step and utilize 4D-DIA holographic scanning technology to achieve in-depth detection of trace samples. Sample volumes can be as low as 100 cells or 5ul of liquid.

We suggest collecting the samples to be delivered in a low-adsorption centrifuge tube and freezing them for transportation at low temperatures.

Quantification of trace plant samples protein based on Cytometric Bead Array (CBA)

CBA is a flow-through protein quantification technique for trace samples, which can quantify soluble proteins. During cell signaling, this method quantifies phosphorylated and apoptosis-related proteins.

Technical advantages

SMID method for preparing trace samples, which is more sample-saving and efficient.

4D-DIA mass spectrometry platform with high sensitivity and more reliable data.

High-depth identification with higher repeatability.

Application Fields

• Study of plant cell heterogeneity.
• Study of proteins in trace amounts of rare plant samples.
• Plant immunity studies.
• Detection of expression of key proteins in signaling pathways.
• Dose-dependent studies of drugs on target proteins.

Data analysis

Statistical analysis of appraisal quantity

Analysis of expression differences

Structural domain analysis

Go function analysis

KEGG analysis

Interaction network and module analysis

Phenotype and omics association analysis

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

For research use only, not for clinical use.