TOP2 | DNA topoisomerase II
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TOP2 | DNA topoisomerase II

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Cat: PA00580
Size: 200 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Vicia faba
Datasheet:

Product Info

Immunogen
The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag is expressed in E.coli and purified by Ni2+ affinity chromatography.
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 500 (IL), 1 : 2000 (WB)
Expected | apparent MW
164 | 170 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Vicia faba
Predicted reactivity
Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrium patens, Pinus sitcHensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox Caterii
Not reactive in
Nicotiana tabacum

Additional information

Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).,Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.This product can be sold containing ProClin if requested.

Description

Type II topoisomerase (EC5.99.1.3) is an enzyme that uses conserved tyrosine as a catalytic residue to catalyze DNA unbinding by creating transient breaks in DNA. This protein is synonymous with At3g23890, ATTOPII, DNA topoisomerase 2,DNA topoisomerase II, F14O13.7, TOP2, topoisomerase II

For research use only, not for clinical use.