Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3
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Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

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Cat: PA00483
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity is observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Datasheet:

Product Info

Immunogen
Pisum sativum TOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Flow Cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
Expected | apparent MW
88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity is observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Predicted reactivity
Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Theobroma cacao, Zostera marina, Vigna radiata, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction

Description

OEP 75 or Toc75; The chloroplast envelope protein of Pisum sativum(pea) is predicted to contain 3 POTRA domains in the N-terminal. The protein transduction channel is considered to be the Toc transposon and assembled into 18 chains ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis, there are five members of the Toc75 family (I-V), with atToc75III being the most closely related to psToc75. In addition, it is structurally related to members of the bacterial surface antigen superfamily, including :OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775)[Envelope biogenesis, outer membrane;YaeT; outer membrane protein assembly complex (TIGR03303);FhaC; hemolysin activating/secreting protein (COG2831)[intracellular transport and secretion]

For research use only, not for clinical use.