TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

TIPs belong to the MIP/ aquaporins family. Alternative names: aquaporin TIP1-1, gamma-tensiplast intrinsic protein, gamma-TIP, aquaporin TIP, gamma-tensiplast intrinsic protein, gamma-TIP, aquaporin TIP, tensiplast intrinsic protein, root specific RB7, tensiplast intrinsic protein 2, gamma-TIP2, Intrinsic protein of tonoplastid induced by salt stress