Thiamine thiazole synthase, chloroplastic (THI1), Recombinant Protein
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Thiamine thiazole synthase, chloroplastic (THI1), Recombinant Protein

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Cat: RP01628
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Thiamine thiazole synthase, chloroplastic (THI1)
Product Gene Name
THI1 recombinant protein
Product Synonym Gene Name
THI1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
FTFDPIKESI VSREMTRRYM TDMITYAETD VVVVGAGSAG LSAAYEISKN PNVQVAIIEQ SVSPGGGAWL GGQLFSAMIV RKPAHLFLDE IGVAYDEQDT YVVVKHAALF TSTIMSKLLA RPNVKLFNAV AAEDLIVKGN RVGGVVTNWA LVAQNHHTQS CMDPNVMEAK IVVSSCGHDG PFGATGVKRL KSIGMIDHVP GMKALDMNTA EDAIVRLTRE VVPGMIVTGM EVAEIDGAPR MGPTFGAMMI SGQKAGQLAL KALGLPNAID GTLVGNLSPE LVLAAADSAE TVDA
Sequence Positions
56-349, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
29,362 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Thionin

NCBI/Uniprot Data

NCBI Accession #
NP_200288.1
NCBI GI #
15239735
NCBI GenBank Nucleotide #
NM_124858.4
NCBI GeneID
835567
NCBI Official Full Name
thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
NCBI Official Symbol
THI1
NCBI Official Synonym Symbols
MBG8.3; MBG8_3; THI1 PROTEIN; THI4; THIAMINE4; THIAZOLE REQUIRING; TZ
NCBI Protein Information
thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
NCBI Summary
Encodes a thiamine biosynthetic gene that has a dual function in thiamine biosynthesis and mitochondrial DNA damage tolerance. It appears to be involved in producing the thiazole portion of thiamine (vitamin B1). A crystal structure of the protein reveals that it forms a 2-ring homo-octamer.
UniProt Gene Name
THI1
UniProt Protein Name
Thiamine thiazole synthase, chloroplastic
UniProt Synonym Protein Names
Thiazole biosynthetic enzyme
UniProt Primary Accession #
Q38814
UniProt Related Accession #
Q38814
UniProt Comments
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. Acts as a positive regulator for the abscisic acid-induced activation of slow type anion channels during stomatal closure by repressing CPK33 kinase activity.

For research use only, not for clinical use.