Succinylated Wheat Germ Agglutinin (WGA), Fluorescein

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Cat: PL00729

Size: 5 mg

Sugar Specificity: N-Acetylglucosamine

Datasheet:

Description

It is reported that this derivative has properties different from natural lectins. Evidence suggests that succinylated wheat germ lectins do not bind to sialic acid residues as the natural form does, but retain specificity for n-acetylglucosamine. Luciferin labeled succinylated WGA has the appropriate amount of fluorescent dye binding to provide the best staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 495nm and the emission maximum is 515nm.

Product Info

Conjugate

Fluorescein

Concentration

5 mg active conjugate/ml

Sugar Specificity

N-Acetylglucosamine

Storage

2-8℃

Excitation

495-500 nm

Emission

514-521 nm

Fluorescence

Green

Application

Immunofluorescence, Glycobiology

Recommended dilution

The recommended concentration range for use is 5-20 µg/ml.

Solution

10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2

For research use only, not for clinical use.