STN8 | Serine/threonine-protein kinase STN8 (chloroplastic)

An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot,For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS). STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.Due to a high background signal with LHCII it is adviced to cut off a membrane below 30 kDa marker.

Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis and st1 congeners in chlamydomonas, is a STN7/ STT7-like protein that does not require state transitions. Arabidopsis mutants lacking the stn8 gene show highly reduced levels of photophosphorylated proteins of photosystem II (Bonardi et al., 2005,Varionen et al., 2005). Alternative name: Protein State Transition 8.