SPY | Spindly (100 µg)

Inquiry

Cat: PA00686

Size: 2 x 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Recombinant SPY protein

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water to each tube

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 500 (WB)

Expected | apparent MW

101,4 kDa

Reactivity

Confirmed reactivity

Recombinant SPY protein

Predicted reactivity

Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.

Description

SPY (SPINDLY), an O-linked N-acetylglucosamine transferase (OGT), is involved in a variety of processes including gibberellin (GA) signaling pathways and biological clocks. It catalyzes the addition of nucleotide-activated sugars directly to the polypeptide through an O-glucoside bond with the hydroxyl group of serine or threonine. As an inhibitor of GA signaling pathway, it inhibits hypocotyl elongation. Alias: Possible UDP-N-acetylglucosamine peptide N-acetylglucosamine transferase.

For research use only, not for clinical use.