Soybean Agglutinin (SBA), Biotinylated

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Cat: PL00694

Size: 5 mg

Sugar Specificity: Galactose, N-Acetylgalactosamine

Datasheet:

Description

SBA preferentially binds to oligosaccharide structures of n-acetylgalactosamine with alpha - or beta - junctions at the end, and to a lesser extent to galactose residues. Binding can be prevented by substitution on the second-to-last sugar, such as the focal point attached to the second-to-last galactose in the type B blood substance. Biotinylated soybean lectin has an appropriate amount of biotin binding, which provides the best staining properties for this lectin. This conjugate contains essentially no unconjugated biotin and is preserved with sodium azide.

Product Info

Conjugate

Biotinylated

Concentration

5 mg active conjugate/ml

Sugar Specificity

Galactose, N-Acetylgalactosamine

Storage

2-8℃; Store frozen for long term storage

Application

Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology

Recommended dilution

For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.

Solution

10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2

For research use only, not for clinical use.