Soybean Agglutinin (SBA), Agarose bound

Agarose bound soy lectins are prepared using affinity purified lectins. Soy lectins are a closely related family of isolates consisting of four subunits of roughly equal size. The molecular weight of the glycoprotein is about 120kda, and the isoelectric point is near pH 6.0. SBA preferentially binds to oligosaccharide structures of n-acetylgalactosamine with alpha - or beta - junctions at the end, and to a lesser extent to galactose residues. Binding can be prevented by substitution on the second-to-last sugar, such as the focal point attached to the second-to-last galactose in the type B blood substance.