SMT1 | Sterol methyltransferase 1

Inquiry

Cat: PA00681

Size: 100 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02, TAIR: At5g13710

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunolocalization (IL), Western blot (WB)

Recommended dilution

1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Expected | apparent MW

38 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera

Not reactive in

Hordeum vulgare, Triticum aestivum, Withania somnifera

Additional information

SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.,SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Description

SMT1 (EC=2.1.1.41), an enzyme involved in steroid biosynthesis, is localized to the endoplasmic reticulum (ER), Boutte et al., 2009. It catalyzes the methyl transfer of C-24 from S-adenosine-methionine to cycloartenol, forming 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and cell expansion areas. Alternate names: cycloartenol-C-24-methyltransferase, 24-sterol-C-methyltransferase 1, protein sterol-methyltransferase 1, protein CEPHALOPOD

For research use only, not for clinical use.