Single B-Cell Sorting Platform

In response to the growing requirement for antibody discovery on difficult targets, CD BioSciences offers a one-stop solution for plant antibody discovery through a powerful and rapid single B-cell sorting platform. We can isolate single B-cells that secrete antigen-specific antibodies in a short period, thereby significantly accelerating clients' experimental progress.

Introduction to the Single B-Cell Sorting Platform

Single B-cell sorting is a microsystem-based screening method. It is based on the principle that the genes encoding the VH and VL regions are amplified directly and only one specific antibody is produced per B-cell. After the isolation of antigen-specific B cells, the IgG heavy and light chain variable region genes are amplified by single-cell PCR. Then expressed in mammalian cells and obtained as biologically active monoclonal antibodies. This platform isolates, sorts, and evaluates B cells, avoiding cell fusion and library construction steps.

Figure 1. Single B cell screening technologies. (Pedrioli, A., & Oxenius, A., 2021)

Single B-cell Isolation Strategies

In recent years, continuous innovations in single-cell isolation techniques have resulted in significant reductions in cell loss and operating costs, and significant improvements in the rate and accuracy of individual cell capture. We offer the following methods to help clients with single-cell sorting.

Figure 2. Current single-cell isolation techniques. (Zeb, Q., et al., 2019)

Fluorescence-activated cell sorting (FACS)

FACS is a widely used technique for isolating single cells. The target cells are labeled with fluorophore-conjugated monoclonal antibodies that recognize specific markers. FACS facilitates random B-cell isolation and antigen-specific B-cell screening based on the expression patterns of specific cell surface markers.

Microfluidics

We have developed a variety of microfluidic chips for the isolation of single cells. Microfluidics does not require labeling, so single-cell separations using microfluidics are based on the intrinsic physical properties of the cells, such as cell size, shape, density, deformability, electronegativity/impedance, and other hydrodynamic properties.

Magnetically activated cell sorting (MACS)

MACS is based on binding magnetic beads to specific proteins on target cells. When a cell suspension is placed in a magnetic field, the magnetic beads will attract the target cells, while unattracted cells will be washed away.

Laser-capture microdissection (LCM)

LCM is a technique for separating cells from tissue samples. It involves identifying the tissue under a microscope and marking the desired section, then using a laser beam to cut the selected area to separate the cells.

Limited dilution

Limited dilution is a method of separating individual cells by diluting cell suspensions. It dilutes the cell suspension to the point where there is only one cell in a single well.

Features of Single B-Cell Sorting Platform

• Integrated high-throughput platforms for antibody selection allow different screens to be performed efficiently.
• It preserves the natural VH and VL pairs, essential for antigen recognition and binding.
• Rare antibodies can be identified directly from B cells.
• Higher efficiency and faster scheduling.

CD BioSciences has decades of experience in plant antibody discovery, antigen-specific single B-cell sorting, and cell manipulation. We utilize our single B-cell sorting platform to provide clients with a one-stop solution for antibody discovery. If you are interested in our platform, please contact us for more details.

For research use only, not for clinical use.