Set of 4 Chlamydomonas anti-Lhc antibodies

Inquiry

Cat: PA01674

Size: 50 µl (Lhcb2, Lhcb5, Lhcbm5), 100 µl (Lhcb4)

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Chlamydomonas reinhardtii

Datasheet:

Product Info

Immunogen

Lhcb2 - BSA-conjugated peptide Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2) Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6) Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein G purified in PBS pH 7.4. (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5).

Format

Lyophilized

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 5000 (Lhcb2,Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)

Expected | apparent MW

25 kDa (Lhcb2) 29 kDa (Lhcb4) 29 | 30 kDa (Lhcbm5) 30| 26 kDa (Lhcb5)

Reactivity

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses

Not reactive in

Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria

Description

The external light-collecting antennas (PSI and PSII) of the photosystem of the green single-celled algae Chlamydomonas reinhardtii consist of a family of pigment-binding proteins (Lhc), which are highly conserved in photosynthetic eukaryotes. Some of the lhc subtypes of Chlamomonas have a high enough similarity to their respective functional homologues in plants to enable specific detection of the conserved peptide domain of plant lhc proteins with antisera.

For research use only, not for clinical use.