Sec21p | Gamma subunit, COP vesicles
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Sec21p | Gamma subunit, COP vesicles

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Cat: PA00382
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
Datasheet:

Product Info

Immunogen
GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 1000 (IF), 1 : 1000 (WB)
Expected | apparent MW
98 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
Predicted reactivity
Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays,
Not reactive in
Nicotiana tabacum, Microsporidia sp.

Additional information

This product can be sold containing proclin if requested,This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot. References describing immunolocalization (IF) and (IG) studies: Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.

Description

Sec21p is a component of the COPI vesicle coating.

For research use only, not for clinical use.