SBP | Sedoheptulose-1,7-bis phosphatase positive control/quantitation standard

The SBPase calibrated protein standard can be used in combination with Agrisera global anti-SBPase antibiodies to quantitate SBPase from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence.  Quantitative western blot: detailed method description and video tutorial.,Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

SBPase (Sedoheptulose-1, 7-HIS phosphatase) is a chloroplast enzyme involved in Calvin cycle carbon reduction as part of carbohydrate metabolism. Source of SBPase standard: The source of SBPase standard is Sphingosphinomonas wilsoni RW1, which is overexpressed in E. coli and carries the n-terminal his6 label.