Sambucus Nigra Lectin (SNA, EBL), Biotinylated
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Sambucus Nigra Lectin (SNA, EBL), Biotinylated

Cat: PL00756
Size: 2 mg
Sugar Specificity: Sialic Acid
Datasheet:

Description

The black Sambucus nigra lectin isolated from the bark of elderberry preferentially binds to the terminal galactose-linked sialic acid in alpha-2,6, and alpha-2,3 chains. Lactose or galactose also inhibit binding to some extent. This lectin appears to bind to sialic acid to n-acetylgalactose, or galactose. It has been reported that SNA can inhibit the synthesis of cell-free proteins. Biotinylated black ginseng lectins bind the appropriate amount of biotin to provide the best staining properties for the lectins. This conjugate contains essentially no unconjugated biotin and is preserved with sodium azide. Note that SNL and SNA are equivalent. Historically, Vector Laboratories has used SNL as an acronym for the lectin sambuus Nigra. SNA, reflecting black ginseng lectin, is commonly used in the scientific community for this product, as shown in B-1305 on the NCFG website. If you have any questions about this matter, please contact us at customerservice@vectorlabs.com or +1-800-227-6666 / +1-650-697-3600.

Product Info

Conjugate
Biotinylated
Concentration
2 mg active conjugate/ml
5 mg active conjugate/ml
Sugar Specificity
Sialic Acid
Storage
2-8℃; Store frozen for long term storage
Application
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended dilution
For most applications, we recommend a freshly prepared working solution of 10 µg/ml in the below buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Solution
10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.

For research use only, not for clinical use.