Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Inquiry

Cat: PL00758

Size: 2 ml

Sugar Specificity: Sialic Acid

Datasheet:

Description

Agarose bound black ginseng lectins are prepared using affinity purified lectins. The black Sambucus nigra lectin isolated from the bark of elderberry preferentially binds to the terminal galactose-linked sialic acid in alpha-2,6, and alpha-2,3 chains. Lactose or galactose also inhibit binding to some extent. This lectin appears to bind to sialic acid to n-acetylgalactose, or galactose.

Product Info

Conjugate

Agarose

Sugar Specificity

Sialic Acid

Storage

2-8℃; Do not freeze

Application

Glycobiology, Affinity Chromatography

Recommended dilution

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-7100. Alternatively, 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 20 mM lactose

For research use only, not for clinical use.