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SAG12 | Senescence-specific cysteine protease SAG12
Cat: PA00755
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare
Datasheet:
Product Info
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana SAG12 protein sequence, UniProt:Q38886, TAIR: AT5G45890
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
38 | 28 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Brassica napus, Hordeum vulgare
Predicted reactivity
Actinidia deliciosa, Brassica rapa, Gossypium hirsutum, Petunia hybrida, Morus notabilis, Nicotiana tabacum, Petunia hybrida, Populus trichocarpa, Ricinus communis, Theobroma cacao
Not reactive in
Citrus sinensis
Additional information
Recommended plant extraction buffer: See Durian et al. (2019). Denature in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) at 70◦C for 5 min
Description
Aging-specific cysteine protease (SAG12) is a cysteine protease that is influenced by cytokinin, auxin and sugar and is involved in developmental age-specific cell death.
For research use only, not for clinical use.